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 E05 Investigative Techniques D008919 +
  E05.393 Genetic Techniques D005821 +
   E05.393.420 Genetic Engineering D005818 +
    E05.393.420.601 Protein Engineering D015202 +
      E05.393.420.601.550 Mutagenesis, Insertional D016254 
      E05.393.420.601.575 Mutagenesis, Site-Directed D016297 
      E05.393.420.601.035 Amino Acid Substitution D019943